
New research strengthens case for age of ancient New Mexico footprints
Researchers used a technique called radiocarbon dating to determine that organic matter in the remains of wetland muds and shallow lake sediments near the fossilized foot impressions is between 20,700 and 22,400 years old. That closely correlates to previous findings, based on the age of pollen and seeds at the site, that the tracks are between 21,000 and 23,000 years old.
The footprints, whose discovery was announced in 2021, indicate that humans trod the landscape of North America thousands of years earlier than previously thought, during the most inhospitable conditions of the last Ice Age, a time called the last glacial maximum.
The age of the footprints has been a contentious issue.
Asked how the new findings align with the previous ones, University of Arizona archaeologist and geologist Vance Holliday, the study leader, replied: 'Spectacularly well.'
Homo sapiens arose in Africa roughly 300,000 years ago and later spread worldwide. Scientists believe our species entered North America from Asia by trekking across a land bridge that once connected Siberia to Alaska. Previous archaeological evidence had suggested that human occupation of North America started roughly 16,000 years ago.
The hunter-gatherers who left the tracks were traversing the floodplain of a river that flowed into an ancient body of water called Lake Otero. The mud through which they walked included bits of semi-aquatic plants that had grown in these wetlands.
Radiocarbon dating is used to determine the age of organic material based on the decay of an isotope called carbon-14, a variant of the element carbon. Living organisms absorb carbon-14 into their tissue. After an organism dies, this isotope changes into other atoms over time, providing a metric for determining age.
'Three separate carbon sources – pollen, seeds and organic muds and sediments – have now been dated by different radiocarbon labs over the course of the trackway research, and they all indicate a last glacial maximum age for the footprints,' said Jason Windingstad, a University of Arizona doctoral candidate in environmental science and co-author of the study published this week in the journal Science Advances.
The original 2021 study dated the footprints using radiocarbon dating on seeds of an aquatic plant called spiral ditchgrass found alongside the tracks. A study published in 2023 used radiocarbon dating on conifer pollen grains from the same sediment layers as the ditchgrass seeds.
But some scientists had viewed the seeds and pollen as unreliable markers for dating the tracks. The new study provides further corroboration of the dating while also giving a better understanding of the local landscape at the time.
'When the original paper appeared, at the time we didn't know enough about the ancient landscape because it was either buried under the White Sands dune field or was destroyed when ancient Lake Otero, which had a lot of gypsum, dried out after the last Ice Age and was eroded by the wind to create the dunes,' Holliday said.
Today, the landscape situated just west of the city of Alamogordo consists of rolling beige-colored dunes of the mineral gypsum.
'The area of and around the tracks included water that came off the mountains to the east, the edge of the old lake and wetlands along the margins of the lake. Our dating shows that this environment persisted before, during and after the time that people left their tracks,' Holliday said.
The area could have provided important resources for hunter-gatherers.
'We know from the abundant tracks in the area that at least mammoths, giant ground sloths, camels and dire wolves were around, and likely other large animals. Given the setting, there must have been a large variety of other animals and also plants,' Holliday added.
The climate was markedly different than today, with cooler summers and the area receiving significantly more precipitation.
'It is important to note that this is a trackway site, not a habitation site,' Windingstad said. 'It provides us a narrow view of people traveling across the landscape. Where they were going and where they came from is obviously an open question and one that requires the discovery and excavation of sites that are of similar age in the region. So far, these have not been found.'

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Daily Maverick
13 hours ago
- Daily Maverick
New research strengthens case for age of ancient New Mexico footprints
Researchers used a technique called radiocarbon dating to determine that organic matter in the remains of wetland muds and shallow lake sediments near the fossilized foot impressions is between 20,700 and 22,400 years old. That closely correlates to previous findings, based on the age of pollen and seeds at the site, that the tracks are between 21,000 and 23,000 years old. The footprints, whose discovery was announced in 2021, indicate that humans trod the landscape of North America thousands of years earlier than previously thought, during the most inhospitable conditions of the last Ice Age, a time called the last glacial maximum. The age of the footprints has been a contentious issue. Asked how the new findings align with the previous ones, University of Arizona archaeologist and geologist Vance Holliday, the study leader, replied: 'Spectacularly well.' Homo sapiens arose in Africa roughly 300,000 years ago and later spread worldwide. Scientists believe our species entered North America from Asia by trekking across a land bridge that once connected Siberia to Alaska. Previous archaeological evidence had suggested that human occupation of North America started roughly 16,000 years ago. The hunter-gatherers who left the tracks were traversing the floodplain of a river that flowed into an ancient body of water called Lake Otero. The mud through which they walked included bits of semi-aquatic plants that had grown in these wetlands. Radiocarbon dating is used to determine the age of organic material based on the decay of an isotope called carbon-14, a variant of the element carbon. Living organisms absorb carbon-14 into their tissue. After an organism dies, this isotope changes into other atoms over time, providing a metric for determining age. 'Three separate carbon sources – pollen, seeds and organic muds and sediments – have now been dated by different radiocarbon labs over the course of the trackway research, and they all indicate a last glacial maximum age for the footprints,' said Jason Windingstad, a University of Arizona doctoral candidate in environmental science and co-author of the study published this week in the journal Science Advances. The original 2021 study dated the footprints using radiocarbon dating on seeds of an aquatic plant called spiral ditchgrass found alongside the tracks. A study published in 2023 used radiocarbon dating on conifer pollen grains from the same sediment layers as the ditchgrass seeds. But some scientists had viewed the seeds and pollen as unreliable markers for dating the tracks. The new study provides further corroboration of the dating while also giving a better understanding of the local landscape at the time. 'When the original paper appeared, at the time we didn't know enough about the ancient landscape because it was either buried under the White Sands dune field or was destroyed when ancient Lake Otero, which had a lot of gypsum, dried out after the last Ice Age and was eroded by the wind to create the dunes,' Holliday said. Today, the landscape situated just west of the city of Alamogordo consists of rolling beige-colored dunes of the mineral gypsum. 'The area of and around the tracks included water that came off the mountains to the east, the edge of the old lake and wetlands along the margins of the lake. Our dating shows that this environment persisted before, during and after the time that people left their tracks,' Holliday said. The area could have provided important resources for hunter-gatherers. 'We know from the abundant tracks in the area that at least mammoths, giant ground sloths, camels and dire wolves were around, and likely other large animals. Given the setting, there must have been a large variety of other animals and also plants,' Holliday added. The climate was markedly different than today, with cooler summers and the area receiving significantly more precipitation. 'It is important to note that this is a trackway site, not a habitation site,' Windingstad said. 'It provides us a narrow view of people traveling across the landscape. Where they were going and where they came from is obviously an open question and one that requires the discovery and excavation of sites that are of similar age in the region. So far, these have not been found.'


Daily Maverick
29-05-2025
- Daily Maverick
Ancient protein from pre-human teeth reveals genetic secrets of human evolutionary tree
Researchers have extracted 2 million-year-old protein remains from ancient pre-human teeth to reveal biological sex and genetic variability. Researchers have extracted 2 million-year-old protein remains from ancient pre-human teeth to reveal biological sex and genetic variability. The teeth are from Paranthropus robustus, an extinct hominin genus that emerged and evolved in Africa between 2.8 and 1.2 Ma. It is considered to be a side branch of our evolutionary tree. It walked on two legs and co-existed with early species of Homo in Africa, possibly interacting. The work, published in the journal Science, marks a significant breakthrough in human evolution studies. It provides some of the oldest human genetic data from Africa and reveals previously undetected genetic variability. 'Because we can sample multiple African Pleistocene hominin individuals classified within the same group, we're now able to observe not just biological sex but, for the first time, genetic differences that might have existed among them,' says the study's co-lead Palesa Madupe. Madupe is a postdoctoral research Fellow at the Globe Institute at the University of Copenhagen and research associate at the Human Evolution Research Institute (HERI) at the University of Cape Town. The researchers used a technique called palaeoproteomics to retrieve ancient protein sequences from the teeth of four Paranthropus robustus fossils recovered from the cave site Swartkrans. Solving the riddle Using state-of-the-art mass spectrometry techniques, they partially reconstructed the ancient enamel protein sequences from the teeth. They found that two of the fossils are male and two are female. But how was this done? Madupe explains: Among the proteins found in tooth enamel, there's one called amelogenin. This protein is unique because its genetic instructions are located on the sex chromosomes: biological females have a version called amelogenin X, while biological males have both amelogenin X and amelogenin Y. 'We used mass spectrometry to detect which protein fragments are present in the fossilised teeth we are analysing. The precise detection of amelogenin Y protein fragments allows us to confidently identify that specimen as belonging to a male individual. 'The challenge comes when we only detect amelogenin X protein fragments, as this could indicate either a female or a male individual whose amelogenin Y is not measured. 'To solve this, we developed a quantitative method for increasing certainty that the lack of amelogenin Y detection proves that those individuals are females.' Eventually, two were identified as male and two as female, just by tiny ancient proteins. Ancient diversity A single genetic variant in another protein, enamelin, was also identified that differentiated the four specimens from one another. Two specimens carried one version of the protein, a third carried another and a fourth specimen appeared to carry both. Their methodology allows for the partial recovery of the amino acid sequences of specific proteins from dental enamel. 'You can imagine this 'amino acid sequence' as a sequence of letters, with each letter corresponding to a specific amino acid [and with 20 possible letters to choose from for each position of the sequence]. An amino acid sequence is usually characteristic of a species; members of the same species will have the same sequence of letters for a protein. 'When we recovered and looked at the enamelin sequence of the four specimens, we saw that the sequences differed at one letter; they had 'a single genetic variant'.' Ioannis Patramanis and Claire Koenig, co-leads from the University of Copenhagen, explained that there are a number of reasons this difference could have occurred. For example, it could be that Paranthropus robustus has a high genetic diversity, or that the four samples belong to different populations or subspecies of Paranthropus, or that we sampled the same species but at different time points in its evolution. 'When studying proteins, specific mutations are thought to be characteristic of a species and, as such, used to identify it. We were thus quite surprised to discover that what we initially thought was a mutation uniquely describing Paranthropus robustus, was actually variable within that group; some individuals had it while others did not,' says Patramanis. The future and DNA HERI co-director Rebecca Ackermann was a senior author on the study, with contributions from co-director Robyn Pickering and several HERI research associates. 'Being able to accurately determine the sex of ancient fossils is a big breakthrough as it allows us to determine whether the variation we see in a sample is due to sexual dimorphism or other factors such as taxonomic diversity,' says Ackermann. 'This has the potential to help us understand sex-related differences in morphology and behaviour. It also provides some control for determining how many species are being sampled. It also may provide direct evidence for understanding the hominin family tree, though this is based on a very small amount of genetic information, so we need to be very cautious in these interpretations. 'Palaeoproteomics does give us insight into genetics, as DNA encodes proteins, so we can work backwards to reconstruct DNA sequences. 'But it's important to remember that the enamel proteome is very small, so this is just a tiny bit of genetic information. At this point, ancient proteins are our only genetic information for deep-time African fossils. 'DNA preservation is poor in African environments, and so far our time depth for understanding human evolution from ancient DNA in Africa is only about 20 thousand years. Only time will tell whether this can be pushed back further!' says Ackerman. DM

IOL News
29-05-2025
- IOL News
Early whalebone tools show inventiveness of prehistoric people
A large projectile point made of gray whale bone from the Duruthy rockshelter, dated between 18 000 and 17 500 years ago, is pictured in Landes, France. Image: Alexandre Lefebvre / REUTERS Will Dunham Artifacts found at archeological sites in France and Spain along the Bay of Biscay shoreline show that humans have been crafting tools from whale bones since more than 20 000 years ago, illustrating anew the resourcefulness of prehistoric people. The tools, primarily hunting implements such as projectile points, were fashioned from the bones of at least five species of large whales, the researchers said. Bones from sperm whales were the most abundant, followed by fin whales, gray whales, right or bowhead whales - two species indistinguishable with the analytical method used in the study - and blue whales. With seafaring capabilities by humans not developing until thousands of years later, the Ice Age hunter-gatherers who made these implements would have been unable to actually hunt whales for their resources in the Bay of Biscay, a gulf of the Atlantic Ocean. "These whales were likely opportunistically acquired from stranded animals or drifted carcasses, rather than actively hunted," said biomolecular archaeologist Krista McGrath of the Autonomous University of Barcelona, co-lead author of the study published in the journal Nature Communications. "The majority of the bones were identified from offshore, deep-water species - such as sperm whale and fin whale - which would have been very difficult to hunt for these prehistoric groups. And there is no evidence from this time period that they had the level of technology that active hunting would have required, like seafaring boats," McGrath said. The 71 whale bone artifacts analyzed by the researchers were found at 27 cave or rock shelter sites. The two oldest ones, both from the bones of fin whales, came from the Spanish Cantabrian sites of Rascaño, dating to about 20 500 years ago, and El Juyo, dating to about 19 800 years ago. The rough age range of the artifacts was from 14 000 years old to more than 20 000 years old, but most were 16 000 to 17 500 years old. The main raw material used to manufacture spear points at the time was antler from reindeer or red deer because it is less brittle and more pliable than land mammal bone. But whale bone offered some advantages, including its large dimensions, with some of the projectile points measuring more than 40 cm long, a size difficult to achieve using antler. "They can be very long and thick, and were probably hafted on spear-style projectiles rather than arrows. They are usually found as fragments, many of which bear fractures related to use, and they were most likely used to hunt the main game animals of the time - reindeer and red deer, horse, bison and ibex," said archaeologist and study co-senior author Jean-Marc Pétillon of the French National Centre for Scientific Research. Bone tools were used by members of the human evolutionary lineage dating back far before our species Homo sapiens emerged more than 300,000 years ago in Africa. The artifacts examined in this study pushed back the oldest-known use of whale bones for tool making by 1 000 to 2 000 years. The objects were previously discovered at the various sites and kept in museum collections. The researchers used modern analytical techniques to determine the species from which the bones came and the age of the artifacts. Humans living in this period of prehistory generally were inland hunters, obtaining most of their subsistence needs from the hunting of large hoofed mammals, Pétillon said. The new findings enhance the understanding of their exploitation of seashore resources, Pétillon added. Previous research had shown that Ice Age people gathered seashells, hunted seabirds and fished for marine fishes as a complement to meat from terrestrial animals. "The new findings tell us that these prehistoric groups were likely very well adapted to these coastal environments, and very likely had deep local ecological knowledge and understanding of their coastal habitats," McGrath said. "Whale bones would have been for more than just making tools. There is evidence for their use as fuel as well - the bones contain large amounts of oil - among other things. And the rest of the whale would also certainly have been used – teeth or baleen depending on the species, meat, skin. A single whale provides a lot of resources," McGrath said. | Reuters