
Scientists discovered a new kind of human with its pinkie bone. Now we have a skull.
Finally, we can put a face on a Denisovan. The "Dragon Man" skull was discovered in Harbin, China in 1933 by a local laborer, but remained hidden away until 2018. A new analysis now finds its very likely to be a Denisovan.
In the summer of 2021, a team of five Chinese researchers stirred up some controversy by suggesting that an unusual skull unearthed in northeastern China belonged to a previously unknown species they thereby officially described as Homo longi, nicknamed 'Dragon Man.' (Both names were inspired by the Long Jiang Dragon River region where it was found.) Soon afterwards, the team was contacted by paleogeneticist Qiaomei Fu of the Institute of Vertebrate Paleontology and Paleoanthropology in Beijing, who asked if she could try and get DNA from the skull.
Back in 2010, she'd been the first to investigate the DNA from a tiny finger bone found in a Siberian cave called Denisova that became world famous because it revealed the existence of a population of hominins not previously known to science, and for which no other fossils existed: the Denisovans.
In two papers published in the journals Science and Cell this week—coauthored with Qiang Ji of Hebei GEO University, an author on the original Homo longi paper—Fu and her team conclude the 'Dragon Man' was likely a Denisovan too. Which is big news, as it makes the stunningly complete skull of 'Dragon Man', also known as the 'Harbin skull', the only Denisovan skull known to science. 'After 15 years, we give the Denisovan a face,' she says. 'It's really a special feeling, I feel really happy.' This illustration depicts how Homo longi may have appeared when they lived during the middle Pleistocene, over 146,000 years ago. Illustration by John Bavaro Fine Art, Science Photo Library
We now know Denisovans had a wide and low face that combined more primitive features, like a prominent brow ridge, with more modern ones, like delicate cheekbones and a relatively flat lower face that does not jut out like it does in other primates and more ancient hominins. Its massive size also suggests a very large body that perhaps helped protect it from brutal winters in northeastern China.
The findings open the door to a better understanding of these ancient hominins and the world they inhabited. 'Having a well-preserved skull like this one allows us to compare the Denisovans to many more different specimens found in very different places,' says paleoanthropologist Bence Viola of the University of Toronto, who was not involved in the new study. 'This means we might be able to compare their body proportions and start thinking about their adaptations to climate, for example.' How dental plaque helped confirm the findings Bulgaria's cultural capital
After she was granted access to the skull, the first thing Fu did was look for DNA, specifically in the teeth and the petrous bone, a dense part of the skull near the inner ear that is known to be the last spot where DNA might survive in a skull that is estimated to be at least 146,000 years old. When that revealed no genetic material, she turned to a different method: extracting proteins. These are usually more hardy than DNA – and because they are what the genes in the DNA code for, they can also provide genetic clues about the DNA that gave rise to them. She was able to collect information from 95 different proteins, four of which are known to differ between Denisovans and other hominins. For three of those, the skull had a Denisovan variant (sometimes in combination with another one on the other chromosome).
Yet Fu still wanted to find DNA to confirm if the skull belonged to a Denisovan. And so she looked in the dental plaque on its single remaining tooth. It was a long shot: while plaque is a very hardy material, researchers more typically find bacterial DNA in it. It's rarer to find the DNA of the owner of the teeth. Against expectations, she did find a tiny amount of DNA there that was human and looked sufficiently old to have belonged to the skull itself, and not one of the people who have handled it since.
(How a molar, jawbone, and pinkie are rewriting human history)
'They may have actually recovered many DNA fragments from me because I studied and handled the specimens so many times,' says paleoanthropologist Xijun Ni, who is based at the same institute and was one of the coauthors of the paper proposing Homo longi as a new species, but was not a coauthor on the current paper. (He is not convinced that the protein analysis is sufficiently specific, nor does he believe the degraded DNA is enough to identify the specimen as Denisovan.)
Fu acknowledges in the paper 'a substantial proportion' of the DNA she found was clearly the result of contamination. But using the established protocols to select only the DNA that is indeed ancient, she found that the tiny amount of DNA that remains, like the proteins, confidently identifies the skull as Denisovan 'It contains 27 gene variants only found in the seven known Denisovan individuals,' says Fu. 'None of these can arise from modern human contamination.'
'The data are quite convincing,' says paleobiologist Frido Welker of the University of Copenhagen in Denmark, who specializes in the analysis of ancient proteins, but wasn't involved in this study. 'The Harbin cranium appears to be a Denisovan.'
Other researchers are convinced as well. Since the description of [the Harbin skull] I was hopeful that we finally had a face for the Denisovans, and these papers prove it,' says Viola who has conducted excavations in Denisova cave. 'It's great that two different methods gave us the same result, this makes me much more confident that this is real.' Denisovans inhabited an even wider range than we thought
These results provoke an unsettled question: Since Denisovans have never been formally described as a species, but Homo longi has, should we now refer to Denisovans as Homo longi?
For some, the answer is clearly yes. 'Assuming the author's claim is true, then Denisovans are a population of Homo longi, just as New Yorkers and Beijingers are both Homo sapiens' says Ni. Paleoanthropologist Chris Stringer of the Natural History Museum in London, who has been collaborating with Ni and others on a new analysis of Chinese hominin fossils, agrees that even though 'it is increasingly likely that Harbin is the most complete fossil of a Denisovan found so far, Homo longi is the appropriate species name for this group.'
But other researchers don't think it's useful to assign separate species names to hominins from this period. 'We ourselves do not use species names for Neandertals or Denisovans,' says paleogeneticist Svante Pääbo of the Max Planck Institute for Evolutionary Anthrology, who leads the lab where Fu first analyzed the Denisovan DNA. 'We do not find it helpful as these are closely related groups that have been shown to mix and have fertile offspring, with each other and with our own direct ancestors. But if a species name is needed, we would simply call them all Homo sapiens.'
(The best evidence yet that Roman gladiators fought lions: a bite mark)
Naming discussions aside, a very exciting discovery remains: a kind of human we once only knew from a pinky bone dug up from a cave now has a face. And we now know this kind of human did not just live in Siberia where the first pinkie bone was found, but across much of East-Asia.
Confidently identifying this fossil will also help researchers make sense of the many other mysterious fossils found across East-Asia, and will encourage them to try and get molecular evidence from those as well. This may also cast a new light on how and when Denisovans and our own ancestors interbred, which is why long after the death of the last direct descendant of the Denisovan 'Dragon Man', some of its genetic material still survives in people alive today.
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Neanderthal fossils have been the subject of study for than a century, but scant details are known about our mysterious Denisovan cousins, and a skull fossil can reveal a great deal. Long in the tooth A laborer in the city of Harbin in northeastern China discovered the Dragon Man skull in 1933. The man, who was constructing a bridge over the Songhua River when that part of the country was under Japanese occupation, took home the specimen and stored it at the bottom of a well for safekeeping. The man never retrieved his treasure, and the cranium, with one tooth still attached in the upper jaw, remained unknown to science for decades until his relatives learned about it before his death. His family donated the fossil to Hebei GEO University, and researchers first described it in a set of studies published in 2021 that found the skull to be at least 146,000 years old. The researchers argued that the fossil merited a new species name given the unique nature of the skull, naming it Homo longi — which is derived from Heilongjiang, or Black Dragon River, the province where the cranium was found. Some experts at the time hypothesized that the skull might be Denisovan, while others have lumped the cranium in with a cache of difficult-to-classify fossils found in China, resulting in intense debate and making molecular data from the fossil particularly valuable. Given the skull's age and backstory, Fu said she knew it would be challenging to extract ancient DNA from the fossil to better understand where it fit in the human family tree. 'There are only bones from 4 sites over 100,000 (years old) in the world that have ancient DNA,' she noted via email. Fu and her colleagues attempted to retrieve ancient DNA from six samples taken from Dragon Man's surviving tooth and the cranium's petrous bone, a dense piece at the base of the skull that's often a rich source of DNA in fossils, without success. The team also tried to retrieve genetic material from the skull's dental calculus — the gunk left on teeth that can over time form a hard layer and preserve DNA from the mouth. From this process, the researchers managed to recover mitochondrial DNA, which is less detailed than nuclear DNA but revealed a link between the sample and the known Denisovan genome, according to one new paper published in the journal Cell. 'Mitochondrial DNA is only a small portion of the total genome but can tell us a lot. The limitations lie in its relatively small size compared to nuclear DNA and in the fact that it is only inherited from the matrilineal side, not both biological parents,' McRae said. 'Therefore, without nuclear DNA a case could be made that this individual is a hybrid with a Denisovan mother, but I think that scenario is rather less likely than this fossil belonging to a full Denisovan,' he added. Mounting evidence The team additionally recovered protein fragments from the petrous bone samples, the analysis of which also suggested the Dragon Man skull belonged to a Denisovan population, according to a separate paper published Wednesday in the journal Science. Together, 'these papers increase the impact of establishing the Harbin cranium as a Denisovan,' Fu said. The molecular data provided by the two papers is potentially very important, said anthropologist Chris Stringer, research leader in human origins at London's Natural History Museum. 'I have been collaborating with Chinese scientists on new morphological analyses of human fossils, including Harbin,' he said. 'Combined with our studies, this work makes it increasingly likely that Harbin is the most complete fossil of a Denisovan found so far.' However, Xijun Ni, a professor at the Institute of Vertebrate Paleontology and Paleoanthropology in Beijing who, along with Stringer, worked on the initial Dragon Man research but not the latest studies, said that he is cautious about the outcome of the two papers because some of the DNA extraction methods used were 'experimental.' Ni also said he finds it strange that DNA was obtained from surface dental calculus but not inside the tooth and petrous bone, given that the calculus appeared to be more exposed to potential contamination. Nonetheless, he added that he thinks it is likely the skull and other fossils identified as Denisovan are from the same human species. The goal in using a new extraction approach was to recover as much genetic material as possible, Fu explained, adding that the dense crystalline structure of dental calculus may help prevent the host DNA from being lost. The protein signatures Fu and her team recovered indicated 'a Denisovan attribution, with other attributions very unlikely,' said Frido Welker, an associate professor of biomolecular paleoanthropology at the University of Copenhagen's Globe Institute in Denmark. Welker has recovered Denisovan proteins from other candidate fossils but was not involved in this research. 'With the Harbin cranium now linked to Denisovans based on molecular evidence, a larger portion of the hominin fossil record can be compared reliably to a known Denisovan specimen based on morphology,' he said. A name and a face for Denisovans With the Dragon Man skull now linked to Denisovans based on molecular evidence, it will be easier for paleoanthropologists to classify other potential Denisovan remains from China and elsewhere. McRae, Ni and Stringer all said they thought it was likely that Homo longi would become the official species name for Denisovans, although other names have been proposed. 'Renaming the entire suite of Denisovan evidence as Homo longi is a bit of a step, but one that has good standing since the scientific name Homo longi was technically the first to be, now, tied to Denisovan fossils,' McRae said. However, he added that he doubts the informal name of Denisovan is going anywhere anytime soon, suggesting it might become shorthand for the species, as Neanderthal is to Homo neanderthalensis. The findings also make it possible to say a little more about what Denisovans might have looked like, assuming the Dragon Man skull belonged to a typical individual. According to McRae, the ancient human would have had very strong brow ridges, brains 'on par in size to Neanderthals and modern humans' but larger teeth than both cousins. Overall, Denisovans would have had a blocky and robust-looking appearance. 'As with the famous image of a Neanderthal dressed in modern attire, they would most likely still be recognizable as 'human,'' McRae said. 'They are still our more mysterious cousin, just slightly less so than before,' he added. 'There is still a lot of work to be done to figure out exactly who the Denisovans were and how they are related to us and other hominins.'