
Company attempting to bring back woolly mammoth turn their sights to the giant moa - a 3.6 metre-tall bird that weighed 230kg
Colossal Biosciences has announced it will attempt to 'de-extinct' a group of birds called the moa, which once lived in New Zealand.
These extraordinary animals included nine species, the largest being the South Island Giant Moa, which stood at 3.6 metres (11.8ft) tall and weighed 230 kg (507 lbs).
Colossal Biosciences will use genes extracted from moa bones to engineer modern birds until they very closely resemble the extinct moa.
This is the same technique that was used to transform grey wolves into animals closely resembling dire wolves last year.
This project will be done in collaboration with the Ngāi Tahu Research Centre at the University of Canterbury and backed by $15 million in funding from Lord of the Rings director Sir Peter Jackson.
Jackson, who has one of the largest private collections of moa bones, says: 'With the recent resurrection of the dire wolf, Colossal Biosciences has also made real the possibility of bringing back lost species.
'There's a lot of science still to be done – but we can start looking forward to the day when birds like the moa or the huia are rescued from the darkness of extinction.'
The nine species of moa were found widely across New Zealand until the arrival of the first Polynesian settlers around 1300 AD.
Within just 200 years, the people who became the Māori had pushed all moa species into extinction through a combination of hunting and forest clearing.
The disappearance of the moa also led to a cascade of changes across New Zealand's isolated island ecosystem.
Less than 100 years after the moa became extinct their main predator, the enormous Haast's eagle, also died out.
But now, advances in genome editing techniques mean Colossal Biosciences may be able to reintroduce the lost moa back to its natural habitat.
The first step is to recreate the genomes of all nine moa species using ancient DNA stored in preserved moa bones.
Colossal Biosciences has already begun this process with visits to caves containing moa deposits within the tribal area of the Ngāi Tahu and hopes to complete all genomes by 2026.
These genomes will then be compared to those of the moa's closest living relatives, the emu and tinamou, to see which genes gave the moa their unique traits.
How will the moa be brought back?
DNA is extracted from moa bones to sequence the moa genome.
The genome is compared to modern species to see which genes make the moa distinct.
CRISPR is used to alter the genome of modern birds to express these target genes.
Edited embryos are placed in a surrogate emu egg to develop.
A bird closely resembling the moa hatches.
A selection of these genes are then inserted into stem cells called Primordial Germ Cell Culture, cells that turn into eggs and sperm, taken from an emu.
Those engineered cells are allowed to develop into male and female gametes and used to create an embryo, which will be raised inside a surrogate emu egg.
Colossal Biosciences has already used these techniques to create mice with the hair of woolly mammoths and wolves very closely resembling extinct dire wolves.
Scientists used the gene editing tool CRISPR to modify the DNA in blood cells from a living grey wolf in 20 places, creating a wolf with long white hair and muscular jaws.
However, recreating this process in bird species poses much greater technical challenges.
Colossal Biosciences admits that creating Primordial Germ Cell Culture for bird species has been a challenge that has eluded scientists for decades.
Likewise, since bird embryos develop inside eggs, the process of transferring an embryo into a surrogate will be completely different from that used for mammals.
Scientists have also raised questions about whether restoring the moa is something that should be pursued at all.
Conservationists say that money would be better spent looking after the endangered species that are already alive.
Others point out that introducing a species which has been gone for over 600 years could have unintended consequences for the ecosystem.
Professor Stuart Pimm, an ecologist at Duke University who was not involved in the study, told AP: 'Can you put a species back into the wild once you've exterminated it there?
'I think it's exceedingly unlikely that they could do this in any meaningful way.'
Professor Pimm adds: 'This will be an extremely dangerous animal.'
However, Colossal Biosciences maintains that their plan to 'rewild' the moa is beneficial for both the environment and the Māori people.
As grazing herbivores, the moa's browsing habits shaped the distribution and evolution of plants over millions of years.
These effects led to significant changes in New Zealand's ecosystems, which Colossal Biosciences argues would be more stable with the moa once again introduced.
Ngāi Tahu archaeologist Kyle Davis, who is working with Colossal Biosciences on the project, says that the project has a deeper ancestral meaning.
During the 14th century, the moa were a vital source of meat for sustenance as well as bones and feathers, which became part of traditional jewellery.
The moa came to have a large role in Māori mythology, symbolising strength and resilience.
Mr Davis says: 'Our earliest ancestors in this place lived alongside moa and our records, both archaeological and oral, contain knowledge about these birds and their environs.
'We relish the prospect of bringing that into dialogue with Colossal's cutting-edge science as part of a bold vision for ecological restoration.'
WHAT DO WE KNOW ABOUT ANCIENT MEGAFAUNA?
Earth was once inhabited by a variety of giant forms of animals that would be recognisable to us today in the smaller forms taken by their successors.
They were very large, usually over 88 pounds (40kg) in weight and generally at least 30 per cent bigger than any of their still-living relatives.
There are several theories to explain this relatively sudden extinction. The leading explanation of around was that this was due to environmental and ecological factors.
It was almost completed by the end of the last ice age. It is believed that megafauna initially came into existence in response to glacial conditions and became extinct with the onset of warmer climates.
In temperate Eurasia and North America, megafauna extinction concluded simultaneously with the replacement of the vast periglacial tundra by an immense area of forest.
Glacial species, such as mammoths and woolly rhinoceros, were replaced by animals better adapted to forests, such as elk, deer and pigs.
Reindeer and Caribou retreated north, while horses moved south to the central Asian steppe.
This all happened about 10,000 years ago, despite the fact that humans colonised North America less than 15,000 years ago and non-tropical Eurasia nearly one million years ago.
Worldwide, there is no evidence of Indigenous peoples systematically hunting nor over-killing megafauna.
The largest regularly hunted animal was bison in North America and Eurasia, yet it survived for about 10,000 years until the early 20th century.
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